Skip to main content
. 2022 Jun 13;2(6):100238. doi: 10.1016/j.crmeth.2022.100238

Figure 5.

Figure 5

Determination of latent and productive infection in different CD4+ T cell subsets

(A) pMorpheus-V5-infected primary human CD4+ T cells from healthy donors stimulated with IL-2 or with IL-2 and α-CD3/CD28 were analyzed using CyTOF to determine levels of NGFR, V5, mCherry, HSA, and p24 in different CD4+ T cell subsets. The bar graphs represent percentage of latent or productive infection of each T cell subsets. The average of two individual donors is shown. Each donor is indicated by a unique symbol and each stimulated group is color coded.

(B) The proportion of latent (yellow) and productive (green) infection of CD4+ T cells upon pMorpheus-V5 infection. CD4+ T cells from 2 donors were stimulated with IL-2 for 3 days. The average of two individual donors is shown.

(C) CD4+ T cells were stimulated with IL2 and α-CD3/CD28 beads for 3 days and infected with pMorpheus-V5. The proportion of latently (yellow) or productively (green) infected cells is depicted. The average of two individual donors is shown.