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. 2022 Jun 13;2(6):100238. doi: 10.1016/j.crmeth.2022.100238

Figure 6.

Figure 6

Validation of latent and productive infection by measuring early activation markers CD69 and HLA-DR in different CD4+ T cell subsets

(A) CD69/HLA-DR expression on latently and productively pMorpheus-V5-infected primary human CD4+ T cells stimulated with IL-2 was determined using CyTOF. Bar graphs represent levels of CD69 and HLA-DR in total CD4+ T cells. Each donor is indicated by a unique symbol. The infection status of each CD4+ T cell subset is color coded in the figure. The average of two individual donors is shown.

(B) CD69/HLA-DR expression on latently and productively pMorpheus-V5-infected CD4+ T subsets stimulated with IL-2 was determined. The levels of CD69+/HLA-DR+ in mock-infected, reporter-negative cells, latently infected, and productively infected CD4+ T cell subsets stimulated were measured using CyTOF. The average of two individual donors is shown.

(C) CD69/HLA-DR expression on latently and productively pMorpheus-V5-infected primary human CD4+ T cells stimulated with IL-2 and α-CD3/CD28 was determined using CyTOF. Bar graphs represent levels of CD69 and HLA-DR in total CD4+ T cells. Each donor is indicated by a unique symbol. Results are from two independent donors.

(D) CD69/HLA-DR expression on latently and productively pMorpheus-V5-infected CD4+ T subsets stimulated with IL-2 and α-CD3/CD28. The levels of CD69/HLA-DR in mock-infected, reporter-negative cells, latently infected, and productively infected CD4+ T cell subsets were measured using CyTOF. The average of two individual donors is shown.