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. 2022 Jun 13;2(6):100238. doi: 10.1016/j.crmeth.2022.100238

Figure 7.

Figure 7

Investigation of immune exhaustion maker Tim-3 in latently and productively infected cells in different CD4+ T cell subsets

(A) Tim-3 expression on latently and productively pMorpheus-V5-infected primary human CD4+ T cells stimulated with IL-2 was determined using CyTOF. Bar graphs represent levels of Tim-3 in total CD4+ T cells. Each donor is indicated by a unique symbol. The infection status of each CD4+ T cell subset is color coded in the figure. Results are from two independent donors.

(B) IL-2 stimulated CD4+ T cells were infected with pMorpheus-V5. The levels of Tim-3POS in mock-infected, reporter-negative cells, latently infected and productively infected CD4+ T cell subsets stimulated were measured using CyTOF. The average of two individual donors is shown.

(C) Tim-3 expression on latently and productively pMorpheus-V5-infected primary human CD4+ T cells stimulated with IL-2 and α-CD3/CD28 beads was determined using CyTOF. Bar graphs represent levels of Tim-3 in total CD4+ T cells. Each donor is indicated by a unique symbol. Results are from two independent donors.

(D) Tim-3 expression on latently and productively pMorpheus-V5-infected CD4+ T subsets stimulated with IL-2 and α-CD3/CD28 beads was determined. The levels of Tim-3POS in mock-infected, reporter-negative cells, latently infected, and productively infected CD4+ T cell subsets were measured using CyTOF. The average of two individual donors is shown.