REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD45 (HI30)-Y89	Fluidigm	Cat#: 3089003B, RRID: AB_2661851
Anti-mCherry 142 Nd, rabbit pAb, coupled in house	Abcam	Cat#: ab167453, RRID: AB_2571870
Anti-human CD45RA (HI100)143Nd	Fluidigm	Cat#: 3143006B, RRID: AB_2651156
Anti-human CD69 (FN50) 144Nd	Fluidigm	Cat#:314401, RRID: AB_2687849
Anti-human CD4 (REA623) 145Nd	Miltenyi Biotec	Cat#: 130-122-283, RRID: AB_2801864
Anti-human CD127 (A019D5) 149Sm	Fluidigm	Cat#: 3149011B, RRID: AB_2661792
Anti-mouse CD24 (M1/69) 150Nd	Fluidigm	Cat#: SKU 3150009B, RRID: AB_2916042
Anti-V5 tag (R960-25) 152Sm, coupled in house	ThermoFisher	Cat#: R960-25, RRID: AB_2556564
Anti-human TIM-3 (F38-2E2) 154Sm	Fluidigm	Cat#: 3154010B, RRID: AB_2893002
Anti-human CD27 (REA499) 155Gd, coupled in house	Miltenyi Biotec	Cat#: 130122295, RRID: AB_2801876
Anti-human Foxp3 (PCH101) 162Dy	Fluidigm	Cat#: 3162011A, RRID: 2687650
Anti-human CD197 (CCR7) (REA546) 167Er, coupled in house	Miltenyi Biotec	Cat#: 130-122-300, RRID: AB_2801881
Anti-human CD3 (REA613) 168Er, coupled in house	Miltenyi Biotec	Cat#: 130-113-138, RRID: AB_2725966
Anti-human CD95 (DX2) 171Yb, coupled in house	Biolegned	Cat#: 305602, RRID: AB_314540
Anti-human HLA-DR 174 Yb, clone REA805	Miltenyi Biotec	Cat#: 130-122-299, RRID: AB_2801880
Anti-human CD45RO (UCHL1) 176Yb, coupled in house	Biolegned	Cat#: 304202, RRID: AB_314418
COULTER CLONE KC57-RD1 165Ho, coupled in house	Beckman Coulter	Cat#: 6604667, RRID: AB_1575989
Anti-human Alexa Fluor 647 CD271 (C40-1457)	BD Pharmingen	Cat#: 560326, RRID: AB_1645403
Anti-V5 tag monoclonal antibody (2F11F7) Alexa Fluor 488	ThermoFisher	Cat#: 37-7500-A488, RRID: AB_2610630
Bacterial and virus strains
pMorpheus-V5 HIV viral vector	This paper	n/a
Biological samples
Human peripheral mononuclear cells, PBMC (Leukopak)	New York Blood Bank	n/a
Chemicals, peptides, and recombinant proteins
Dextran	Millipore Sigma	Cat#: 9004-54-0
Bovine Serum Albumin (BSA)	ThermoFisher	Cat#: A1113803
Human recombinant IL-2	NIH AIDS Reagent	Cat#: 136
Dynabeads human T- Activator CD3/CD28	Thermo Fisher Scientific	Cat#: 11161D, AB_2916088
Cell-ID Intercalator-103 Rh	Fluidigm	Cat#: 201103A
Cell-ID IdU-127I	Fluidigm	Cat#: 201127
Cell-ID Intercalator- 191 Ir	Fluidigm	Cat#: 201192A
Cell-ID Intercalator- 193 Ir	Fluidigm	Cat#: 201192A
Critical commercial assays
Human CD4+ T cell isolation Kit	Miltenyi Biotec	Cat#: 130-096-533, RRID: AB_2916089
LIVE/DEAD Fixable dead cell stain kits	Invitrogen	Cat#: L10119
Experimental models: Cell lines
Human: HEK293T	ATCC	ATCC#: CRL-3216, RRID: CVCL_0063
Oligonucleotides
Oligonucleotides, primers and probes used in this study	This paper	See Table S2
Recombinant DNA
pMorpheus-V5	This paper	n/a
Software and algorithms
FlowJo	FlowJo, LLC	http://www.flowjo.com, RRID: SCR_008520
PRISM 9.0	Graphpad	http://graphpad.com, RRID: SCR_005375
Cytobank	(Kotecha et al., 2010)	http://www.cytobank.org, RRID: SCR_014043