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. 2022 Feb 28;37(3):358–369. doi: 10.1016/j.virs.2022.02.011

Fig. 1.

Fig. 1

Repression of cellular Pol II-dependent transcription by HCMV RNA2.7. A General alteration of genome transcription in cells infected with HAN or HANΔRNA2.7 (MOI: 1.0). Cellular transcripts changed by infection are sorted in Venn diagram. B Comparison results on diseases and biofunctions of HAN or HANΔRNA2.7 altered transcripts. Their effects are indicated with Z-score: an active effect is indicated with Z-score > 2.0; a suppressive effect is indicated with Z-score < −2.0. C Venn diagram showing genes involved in host gene activation. HANΔRNA2.7 specific genes were analyzed and categorized into transcription from Pol II promoter. D Activation of Pol II-dependent regulators. Activities of transcriptional regulators were modified by RNA2.7. The effects are indicated with Z-score: Z-score > 2.0 indicates a significantly transcriptional activation of downstream genes; 2.0 ​> ​Z-score > 0 indicates a transcriptional activation of downstream genes; 0 ​> ​Z-score indicates a transcriptional repression of downstream genes. E Western blot analysis for pCDK9, Pol II and its two phosphorylated forms (Pol II S2 and Pol II S5) in cells infected with HAN or HANΔRNA2.7 ​at different time points. IE1 and GAPDH were measured as controls. Data are presented as mean ​± ​SEM. Statistical analysis was performed by Student's t-test. ∗∗∗P ​< ​0.0001.