Fig. 4.

Effects of RNA2.7 on viral DNA replication. A Growth curves of HAN and HANΔRNA2.7 in HELF cells. Growth curves were performed in quiescent HELF cells infected with HAN or HANΔRNA2.7 ​at an MOI of 0.1 and 3. Supernatant samples were collected at 1, 3, 6 and 9 dpi. Titers were determined by TCID₅₀. HAN is indicated with solid lines, and HANΔRNA2.7 is indicated with dashed lines. Data are averages from three independent experiments, and standard errors are indicated. B Up-regulation of viral replication by Cdt1 and Cdc6 knockdown. Evaluated siRNA specific to Cdt1 and Cdc6 were transfected into HELF cells. Cells transfected with siRNAs specific to Cdt1 or Cdc6 were infected with HANΔRNA2.7 (MOI ​= ​1.0). Cells transfected with siRNA negative control were infected with HAN or HANΔRNA2.7. Viral DNA levels were quantified at 2, 24 and 96 hpi. Relative viral DNA levels are presented as mean ​± ​SEM. Statistical analysis was performed by Student's t-test. ∗P ​< ​0.05; ∗∗P ​< ​0.01. TCID₅₀, standard median tissue culture infective dose.