Figure 7.

Comparison of stem cell morphology, number, and size and cell cycle regulation between JcFT^OE and control (CK) plants. (A) Stem cross sections of JcFT^OE and control plants (The values in the central boxes represented the diameter size of the samples, scale bar = 1 mm). (B) Average size of pith cells in JcFT^OE and control plants (The values represented mean, the vertical bars indicated standard deviation, n = 66, ^**p < 0.01). (C,D) Stem cross sections (same magnification) of JcFT^OE and control plants at 49 days after seeding (scale bar = 100 μm). Double arrows indicate thickness of the cortex, and straight lines indicate larger cells in the cortex. (E,F) Stem longitudinal sections (same magnification) of JcFT^OE and control plants at 49 days after seeding (scale bar = 100 μm). (G) BiNGO annotated overrepresented GO (gene ontology) terms associated with cell cycle regulation. Color of a node represents the corrected p-value, with the scale ranging from yellow (p = 0.01) to dark orange (p = 0.01 × 10⁻⁵) and size of a node indicates the number of genes involved. Different colors of arrows suggested different types of enriched pathways. (H) Expression heat map of differentially expressed genes (DEGs) associated with DNA-dependent DNA replication initiation. Control samples: T1, T2, T3; JcFT^OE samples: T4, T5, T6. Blue indicates a decrease in gene expression; red indicates an increase in gene expression. (I) Reverse-transcription qPCR of 10 DEGs associated with DNA-dependent DNA replication initiation (bars represented gene expression mean and standard error of mean, n = 3, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, and the corresponding gene accession number was under the bars.). Epi: epidermis; Co: cortex; Ph: phloem; Xy: xylem; Pi: pith.