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Huaier induces ICD of tumor cells. (A) Flow cytometry analysis of surface expression of CRT in breast cancer cells treated with Huaier. (B) ATP secretion was detected in the media of TNBC cells treated with Huaier at indicated time and concentration. (C) Concentrated supernatants, membranes and cell lysates were collected for western blot. (D) CRT translocation was assessed by immunofluorescence staining in MDA-MB-231 and 4T1 cells. Scale bars: 10 μm *p < 0.05; **p < 0.01.
