FIGURE 2.

Huaier enhances activation of DC cells. (A) The experimental design and cell culture process of bone marrow derived DC cells. (B) The populations of activated DCs (CD86^high CD11c⁺) were presented via the FlowJo analyses. (C) Scheme of the vaccination assay in vivo. (D) 4T1 cells were pretreated with Huaier, or freeze-thawed, followed by inoculated s.c. into BALB/c mice as a vaccine (n = 6 for each group). A week later, mice were rechallenged with live 4T1 cells. (E) Individual tumor growth curves of tumor-bearing mice in three groups. s.c., subcutaneously; *p < 0.05; **p < 0.01.