Skip to main content
. 2022 Jun 16;10:913824. doi: 10.3389/fcell.2022.913824

FIGURE 7.

FIGURE 7

CircCLASP1 enhanced the Huaier-induced ER stress via directly interacting with PKR. (A) Silver staining image of proteins pulled down by circCLASP1. (B) RNA pull-down and western blotting analyses with sense and anti-sense of circCLASP1 evaluated the physical interaction between circCLASP1 and PKR. (C) Schematic representation of the experimental protocols of RIP assay. (D) RIP assay was performed using MDA-MB-231 cell lysates and PKR antibodies. Enrichment of circCLASP1 was measured via qRT-PCR analysis. (E) FISH and immunofluorescence assays estimated the colocalization of circCLASP1 and PKR in MDA-MB-231 and MDA-MB-468 cells. Scale bars: 10 μm. (F) CircCLASP1 enhanced the stabilization of PKR in MDA-MB-231 and MDA-MB-468 cells. (G) Huaier increased the protein level of PKR. (H) CircCLASP1 enhanced the regulation effects of Huaier on PKR. (I) CircCLASP1 enhanced the regulation effects of Huaier on phosphorylation of ATF4. (J) Huaier induced immunogenic cell death by circCLASP1/PKR/eIF2α axis in TNBC. *p < 0.05; **p < 0.01.