FIG. 3.

Cell-free biotransformation of morphine at various ratios of MDH to MR in the presence and absence of STH. The transformations were performed without (A, C, and E) or with (B, D, and F) 1.25 U of STH per ml. MDH and MR were used as the ratios 1:1 (1.25 U of each per ml) (A and B), 5:1 (6.25 U of MDH per ml and 1.25 U of MR per ml (C and D), 1:5 (1.25 U of MDH per ml and 6.25 U of MR per ml (E and F). The cofactors NADPH and NAD⁺ were supplied at a concentration of 0.2 mM.