TABLE 1.
Enzyme activities obtained from expression constructs in E. coli JM109a
| Construct in E. coli JM109 | Sp act (U/mg) of:
|
||
|---|---|---|---|
| MDH | MR | STH | |
| pMORAB5 | 5.76 ± 1.02 | 1.92 ± 0.33 | — |
| pMORAB5/pPFSTH4 | 4.19 ± 0.07 | 1.60 ± 0.08 | 0.58 ± 0.01 |
| pMORB3-AC80S · K244M | 0.030 ± 0.001 | 0.84 ± 0.05 | — |
| pMORB3-C80S · K244M/pPFSTH4 | 0.040 ± 0.004 | 1.03 ± 0.07 | 0.45 ± 0.05 |
a
Figures shown are the averages of triplicate measurements ± the standard deviations. Crude cell extracts were prepared as described in the text. Units of enzyme activities are as defined in the text. —, no detectable activity.