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. 2022 Jun 27;54(6):834–847. doi: 10.1038/s12276-022-00791-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a MTS assays were performed to measure the proliferation (OD 490 nm) of KYSE150 and TE-11 cells with TAS1 knockdown (KD) compared with control cells (n = 3). b BrdU incorporation assays (OD 450 nm) of KYSE150 and TE-11 cells with TAS1 KD compared with control cells (n = 3). c Statistical analysis of the cell cycle distribution (%) of KYSE150 and TE-11 cells with TAS1 KD compared with control cells. d Statistical analysis of the migration and invasion rates (%) of KYSE150 and TE-11 cells with TAS1 KD (n = 3). e Statistical analysis of the migration and invasion rates (%) of KYSE30 and TE-15 cells with TAS1 overexpression (OE) (n = 3). f MTS assays were performed to measure the proliferation of KYSE30 and TE-15 cells with TAS1 OE compared with control cells (n = 3). The data are presented as the mean±S.D. values. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.