Fig. 4.

Comparison of the results of the neutralization assay and ELISA. A, B Neutralizing activity against type I IFNs was compared between type I IFN concentrations of 100 pg/mL and 10 ng/mL stimulated by IFN-α2 (A) or IFN-ω (B). C–F aAbs to type I IFNs by ELISA were compared with naAbs by the neutralization assay at concentrations of 10 ng/mL IFN-α2 (C), 10 ng/mL IFN-ω (D), 100 pg/mL IFN-α2 (E), and 100 pg/mL IFN-ω (F). The cutoff value of ELISA was 0.5 (O.D.). In neutralization assay, samples showing less than 15% of luciferase activity were defined as having neutralization activity