Fig. 2. Enteric viruses replicate acutely and persistently in SGs and are transmitted through saliva.
a, Schematic of saliva collection. b,c, Saliva collected from EDIM-infected (b) or MNV-1-infected (c) mice probed with anti-VP-6 and anti-VP1, respectively (n = 3, five animals per experiment). d, MNV-1 titres in saliva (n = 3, three animals per experiment; each dot represents an experiment). e, WU23, MNV-4 and MNV-3 titres in saliva (n = 3, four animals per experiment; each dot represents an experiment). f, Schematic of mouse SMGs. g,h, MNV-1 titres in pup (n = 4) (g) and adult (h) SMGs (n = 3). i,j, EDIM replication in pup (i) (n = 4) and adult (j) SMGs (n = 3). g–j, Each data point represents one animal, seven animals per experiment. k, WU23, MNV-4 and MNV-3 titres in SMGs (n = 3, five animals per experiment; each dot represents one animal). l–n, Replication in the SGs, proximal colon, Peyer’s patches and spleen of adult mice inoculated with WU23 (l), MNV-4 (m) and MNV-3 (n) (n = 3, five animals per experiment; each dot represents data from one animal). o, Schematic of oral inoculation with infected saliva. p, Viral replication in the small intestines of pups orally inoculated with infected saliva (n = 3, each dot represents one animal, total six animals). Pups were also inoculated with uninfected saliva for baseline reference. Data are the mean ± s.e.m. d,e,g–j,k, Two-tailed unpaired t-test. Statistical information is in Supplementary Table 4. For gel source data, see Supplementary Figure 1. The input for d,e,g–k,l–n, is 6 hpi.