Fig. 3. Murine norovirus and rotavirus replicate in the epithelial and immune cells of SMGs.
a,b, MNV-1 titres in epithelial (EpCAM+) and immune (CD45+) cells sorted from the SMGs of inoculated pups (a) and adults (b) (n = 3, each dot represents data from two animals, six animals in total). c–f, Immunostaining of SMGs from uninoculated and MNV-1-inoculated pups with anti-NS4, anti-J2 (dsRNA), anti-EpCAM and anti-CD45. MNV-1 replication in acinar (arrows and box 1) and immune cells (box 2). g, Schematic of salivary duct structure. h, EDIM replication in EpCAM+ and CD45+ cells sorted from the SMGs of inoculated pups (n = 3, each dot represents two animals, six animals in total). i,j, Immunostaining of SMGs from uninoculated (i) and EDIM-inoculated (j) pups with anti-NSP5, anti-CD45 and anti-EpCAM. EDIM replication in immune (box 1) and ductal cells (arrows and box 2). k, Cd300lf expression in SMGs (n = 3, three adults and three pups per experiment). RAW246.7 and HeLa cells were used as positive and negative controls, respectively. l, MNV-1 titres in the SMGs of inoculated Cd300lf−/− and Cd300lf+/+ mice (n = 3, each dot represents one animal, five animals per experiment for each group). m, MNV-1 titres in the intestines of inoculated SG-removed or SG-intact adult mice (n = 3, each dot represents one animal, six animals per experiment for each group). a,b,h,k, Gating strategies are depicted in Extended Data Fig. 3. Data are the mean ± s.e.m. l,m, Two-tailed unpaired t-test between two groups for each time point. Statistical information is given in Supplementary Table 4. m, Input is 6 hpi. All micrograph experiment reproducibility information is found in the Methods.