Table 1.

Identification of the viruses found by total RNA seq in P. castaneae isolate VN999 and a virus found in VN1008 by sRNA seq and the most similar sequences in GenBank based on a BlastX search.

Virus name	Acronym1	Length2	GenBank3	Putative family/Order4	BlastX best hit5	E value	QC(%)6	I (%)7	Identified by BlastX search of Velvet contigs (yes/no)
Phytophthora castaneae RNA virus 1	PcaRV1	6,337	MZ269516	Megabirnaviridae	RdRp Charybdis toti-like virus (DAZ87259.1)	2e−59	34	29.08	yes
Phytophthora castaneae RNA virus 2	PcaRV2	2,891	MZ269517	Narnaviridae	RdRp Sanya narnavirus 11 (UHM27569.1)	2e−102	81	32.20	yes
Phytophthora castaneae RNA virus 3	PcaRV3	5,470	MZ269518	Totiviridae	putative RdRp polymerase Totiviridae sp. (UHS72506.1)	0.0	39	50.75	yes
Phytophthora castaneae RNA virus 4	PcaRV4	6,884	MZ269519	“Fusagraviridae”	ORF2 Bremia lactucae associated fusagravirus1 (QIP68010.1)	7e−47	31	28.15	no*
Phytophthora castaneae RNA virus 5	PcaRV5	2,182x	ON131873	Endornaviridae	hypothetical polyprotein Diatom colony associated dsRNA virus 15 (YP_009552081.1)	2e−123	99	34.82	yes
Phytophthora castaneae negative-stranded RNA virus 1	PcaNSRV1	8,345	MZ269515	Bunyavirales	polyprotein Phytophthora cactorum bunyavirus 1 (QUA12643.1)	0.0	97	57.16	yes

¹Acronym of Phytophthora castaneae RNA virus 1–5 and Phytophthora castaneae negative-stranded RNA virus 1.

²Length, virus sequence length obtained through Trinity assembly.

³GenBank accession number of each virus.

⁴Putative Family/Order, placement of the viruses according to their genome organization and phylogenetic analysis.

⁵The most similar virus according to BlastX search when the whole virus sequence is blasted against the (nr)protein sequence database in the online version of NCBI BlastX (date of the last search: 01.04.2022).

⁶QC, Query cover.

⁷I, Identity.

*PcaRV4 was identified by BlastX search of Velvet contigs only in one isolate (VN1012).

^xSequence of PcaRV5 is surely partial. The date of the last BlastX search 01.04.2022.