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. 2022 Jun 16;13:922689. doi: 10.3389/fmicb.2022.922689

FIGURE 7.

FIGURE 7

Evaluation of the adhesion capacity of Lc. paracasei SP5 onto the human colon cancer adenocarcinoma cell line, HT-29. (A,B) Representative photos from confocal fluorescent microscopy showing the adhesion capacity of Lc. paracasei SP5 or Lc. rhamnosus GG. Panel A represents a zoomed in image of a 40x photo, edited by ImageJ (version 1.53f51), while panel B represents a photo of the original magnification (40×). Bacteria are stained with CFSE (green), eukaryotic nuclei (blue) and cell membranes (red) are stained with Hoescht and CellBrite Red Cytoplasmic Membrane Dye, respectively (scale bar, 5 μm). (C) Gene matrix depicting the presence or absence of adhesion-related proteins in the genomes of Lc. paracasei SP5 and Lc. rhamnosus GG, annotated by PGAP. (D) Determination of attached bacterial counts (CFU/mL) of Lc. paracasei SP5 (black bar) or Lc. rhamnosus GG (grey bar) after 4 h co-incubation with HT-29 monolayers. Results are presented as mean ± standard deviation.