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. 2022 Jun 29;10(6):e004875. doi: 10.1136/jitc-2022-004875

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Activation of eIF4E is responsible for both SP20H knockout and TNF-α stimulation induced B7-H3 translation. (A) Total protein expression of B7-H3, phospho-p38, and phospho-eIF4E in SB203580 treated SP20H knockout or TNF-α pretreated SK-OV-3 cells. (B) Changes of p38 MAPK-eIF4E signaling and B7-H3 expression in SK-OV-3 cells transduced with the indicated sgRNAs under TNF-α stimulation. (C) The expression levels of B7-H3 after interfering with eIF4E in the SP20H knockout SK-OV-3 cells. The values represent the mean ratios of B7-H3^high/B7-H3^low population of triplicate measurements±SD. (D) Schematic illustration of two ways to induce B7-H3 expression via the p38 MAPK- eIF4E signaling.