Figure 3.

SrrG sRNA sequence and its effect on sagA expression.

(a) Position of SrrG sRNA. The putative −10 and/or −35 promoter sequences are indicated by black boxes and the putative rho-independent (intrinsic) terminators are highlighted by inverted blue arrows. The green arrow indicates the direct repeats region. The transcriptional product of SrrG was determined by 5′ and 3′ RACE. (b–c) sagA expression post introduction of SrrG sRNA into (b) NIH960 and (c) SDSE80srrG was determined by RT-PCR. sagA expression in each strain was analysed by RT-PCR. Columns represent the relative sagA mRNA levels in each strain. The expression level of SDSE80/pAT18 strain is shown as 1. Error bars displayed as mean +/- standard deviation (±S.D.) (n = 4). (d) Haemolysis in the srrG and csrS mutants after 5 hours using Columbia agar plates with 5 % sheep blood.