TABLE 2.
Respiratory electron transport rates in isolated intact mitochondria from intact wild type Arabidopsis leaves.
| A | |||||||||
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| Maximal COX activity | Percentage of intact mitochondria | ||||||||
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| WT | OEX2-5 | WT | OEX2-5 | ||||||
| 142.9 ± 7.9 | 166.2 ± 9.8* | 85.4 ± 3.2 | 89.8 ± 1.3 | N=24 | |||||
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| B | |||||||||
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| Genotype | CI substrates | ADP | Oligomycin | FCCP | |||||
|
| |||||||||
| WT | 23.0 ± 2.5 | 18.6 ± 1.1 | 1.0 ± 0.5 | 20.4 ± 3.8 | |||||
| OEX2-5 | 22.0 ± 2.5 | 22.5 ± 5.2 | 1.4 ± 0.3 | 14.0 ± 4.1 | N=4 | ||||
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| C | |||||||||
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| Succinate | Rotenone | ADP | Oligomycin | FCCP | |||||
|
| |||||||||
| WT | 23.1 ± 3.4 | 26.2 ± 3.0 | 41.0 ± 3.3 | 26.2 ± 4.6 | 32.6 ± 3.4 | ||||
| OEX2-5 | 22.8 ± 4.2 | 24.2 ± 2.6 | 33.4 ± 3.9 | 27.5 ± 3.5 | 24.3 ± 4.6 | N=15 | |||
(A) Maximal respiration rates through complex I (CI) cytochrome C oxidase (COX). COX activity expressed as nmol O2 * min–1 * mg protein–1; (B) electron transport through respiratory complex I (CI) in the absence or presence of ADP, oligomycin or p-triflouromethoxyphenylhydrazone (FCCP); (C) electron transport through respiratory complex II.