Figure 2.

Cancer cell line supernatant evokes orofacial nociceptive behavior in male mice. (A) Experimental timeline for the cell line supernatant model of oral cancer pain. Adult C57BL/6 male mice were trained in a dolognawmeter over 4 weeks or until a consistent baseline was achieved. Three additional baseline trials were completed and then mice underwent 3 consecutive injections of cell line supernatant followed by assessment in the dolognawmeter (Postinjection Gnaw-Time Measurement). There was no change in gnaw-time between groups; therefore, the mean of the 3 baseline trials for each group were calculated and analyzed as a percent change. (B) Pooled data for Pre-Treatment and Post-treatment trials as well as (C) individual values for each mouse in the Post-Treatment Trial are represented as a percent change from the average baseline gnaw-time (two-way ANOVA, ** P < 0.01). ANOVA, analysis of variance.