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Effects of pH on enzyme activities. The aldehyde reduction activity (open symbols) with heptanal and the alcohol dehydrogenation activity (solid symbols) with heptanol were assayed at various pHs. In the case of alcohol dehydrogenation activity, NADP+ was used as the cofactor. Symbols: triangles, acetate buffer; squares, sodium phosphate buffer; circles, Tris-Cl buffer; diamonds, glycine-NaOH buffer.
