TABLE 1.

Purification of alcohol dehydrogenase from Acinetobacter sp. strain M-1

Purification step	Total activity (U)	Total protein (mg)	Sp act (U · mg−1)	Purification (fold)	Yield (%)
Soluble fraction	820	1,900	0.43	1	100
Butyl-Toyopearl	630	940	0.67	1.6	77
Phenyl-Sepharose	400	84	4.8	11	49
Dye Matrex Red A	350	4.9	72	170	43
Superdex 200	130	0.26	490	1,200	16
Q-Sepharose	24	0.043	560	1,300	3.0