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. 2022 Jun 21;3(6):100541. doi: 10.1016/j.xcrm.2022.100541

Figure 2.

Figure 2

Expression of PDE5 in the esophagus and manipulation of CAF differentiation in vitro by PDE5i

(A) PDE5 expression analysed by IHC of normal esophagus and EAC. PDE5 expression is localized to both tumor cells and the surrounding stromal tissue. Scale bars, 200 μm.

(B) Western blot and ICC for PDE5 expression in three cancer cell lines (MFD-1, FLO-1, and OE33) and five matched normal (NOF)/cancer (CAF) primary esophageal fibroblasts, all normalized to Hsc70.

(C) NOFs treated with TGF-β1 ± 50 μM vardenafil for 72 h. TGF-β1-treated NOFs express higher α-SMA, and vardenafil pre-treatment abrogated the TGF-β1-induced expression of α-SMA.

(D) CAFs treated with 50 μM vardenafil for 72 h reduced α-SMA expression 50% by western blot and ICC. One-way ANOVA, p values: ∗p < 0.05, ∗∗∗∗p < 0.0001. Results are representative of three independent experiments. See also Figure S1.