Figure 7.

Efficacy study of PDX mouse models treated with chemotherapy alongside PDE5i

(A) Tumor volumes of PDX models treated with saline for injection (vehicle), CX alone, or CX + PDE5i vardenafil (Var) or tadalafil (Tad) (n = 15 per treatment arm).

(B and C) Example images of Untreated, CX, CX + Vardenafil, and CX + Tadalafil treatment arms in mouse PDX models are presented: IHC stained with (B) anti-α-SMA and (C) anti-periostin antibodies.

(D and E) CAF differentiation was quantified by thresholding of digital whole-slide images from PDX models to measure total area stained for α-SMA (D) and periostin (E).

See also Figures S3 and S4 and Table S4. Box and whisker plots represent the IQR of log2 expression of PDE5A. Central line = median, outliers are individual points outside of whiskers.