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. 2022 Jun 14;3(6):100658. doi: 10.1016/j.xcrm.2022.100658

Figure 4.

Figure 4

Depletion of AMMO1-KO-insensitive antibodies from pooled plasma

(A–D) The binding of AMMO1 (A), CL40 (B), CL59 (C), and E1D1 (D) binding to gH/gL and gH/gL-KO (gH K73W,Y76A/gL) were measured using biolayer interferometry.

(E–H) Antibodies were depleted from pooled plasma collected following three immunizations with gH/gL or gH/gL nanoparticles using gH/gL-KO conjugated magnetic beads. Pre- and post-depletion plasma samples were assayed for binding to gH/gL and gH/gL-KO by ELISA as indicated. Each data point represents mean, and error bars represent the standard deviation of two technical replicates.

(I–L) The ability of plasma pre- and post-depletion to neutralize EBV infection was measured in B cells and epithelial cells. Each data point represents the mean, and error bars represent the standard deviation of two technical replicates.