REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Recombinant AMMO1	Snijder et al., 2018	N/A
Recombinant CL40 with human constant regions	Singh et al., 2020	N/A
Recombinant CL59 with human constant regions	Singh et al., 2020	N/A
Recombinant E1D1 with human constant regions	This study	N/A
hCD20- BV786	BD Biosciences	Cat# 743611; RRID:AB_2741622
hCD45-FITC	ThermoFisher	Cat# 5010066; RRID:AB_1907394
mCD45-APC	Biolegend	Cat# 103112; RRID:AB_312977
hCD33-PE	BD Biosciences	Cat# 555450; RRID:AB_395843
hCD19- BV711	Biolegend	Cat# 302246; RRID:AB_2562065
hCD4- AF700	ThermoFisher	Cat# 56-0048-82; RRID:AB_657741
hCD8-BV421	BD Biosciences	Cat# 562429; RRID:AB_11153676
mCD16/32	Biolegend	Cat# 101302; RRID:AB_312801
Rabbit anti-His tag	Sigma Aldrich	Cat# SAB5600227
Goat anti-mouse IgG-HRP	SouthernBiotech	Cat# 1033–05; RRID:AB_2737432
Goat anti-human IgG-HRP	SouthernBiotech	Cat# 2010–05; RRID:AB_2795564
Goat anti-human IgG-HRP	Jackson ImmunoResearch	Cat# 115-035-008; RRID:AB_2313585
Goat anti-human IgG	SouthernBiotech	Cat# 1030–01; RRID:AB_2794290
Goat Anti-Mouse IgG, Human ads-HRP	SouthernBiotech	Cat# 1030–05; RRID:AB_2619742
Bacterial and virus strains
EBV B95.8/F	Delecluse et al., 1998	N/A
EBV AKTA-GFP	Molesworth et al., 2000	N/A
Biological samples
human CD34-enriched PBSCs	Co-operative Center for Excellence in Hematology, Fred Hutchinson Cancer Research Center	N/A
Chemicals, peptides, and recombinant proteins
Platinum Super-Fi II DNA polymerase	ThermoFisher	Cat# 12361010
In-fusion HD cloning kit	Takara Bio	Cat# 638920
Polybrene	Millipore Sigma	Cat# TR-1003-G
293Freestyle Media	ThermoFisher	Cat# 12338018
Phosphate Buffered Saline	Corning	Cat# 21-040-CM
Protein A Agarose	GoldBio	Cat# P-400-5
Pierce IgG Elution Buffer	ThermoFisher	Cat# 21004
Ni-NTA resin	ThermoFisher	Cat# 88221
Galanthus Nivalis Lectin Agarose	Vector Laboratories	Cat# AL-1243-5
disuccinimidyl suberate	Thermofisher	Cat# 21555
synthetic lipid A in squalene emulsion SLA-SE	Infectious Disease Research Institute	N/A
Sigma Adjuvant System	Sigma Aldrich	Cat# S6322
Pierce Protein A/G Binding Buffer	ThermoFisher	Cat# 54200
Pierce Protein A/G resin	ThermoFisher	Cat# 20422
GeneJuice Transfection Reagent	Sigma Aldrich	Cat# 70967
293-Free Transfection Reagent	Millipore Sigma	Cat# 72181
0.25% trypsin	ThermoFisher	Cat# 25200056
10% Formalin	Millipore Sigma	Cat# HT501128
eBioscience Fixable Viability Dye eFluor 506	ThermoFisher	Cat# 65-0866-18
SureBlue Reserve TMB Microwell Peroxidase substrate	SeraCare	Cat# 5120–0081
EZ-Link NHS-PEG4-Biotin Kit	ThermoFisher	Cat# 21330
Zeba Spin Desalting Columns 40K MWCO	ThermoFisher	Cat# 87766
NeutrAvidin Protein	ThermoFisher	Cat# 31000
Streptavidin Magnetic Beads	New England BioLabs	Cat #S1420S
Critical commercial assays
DNeasy Blood & Tissue Kit	Qiagen	Cat# 69504
2×QuantiTect Probe PCR Master Mix	Qiagen	Cat# 204343
Anti-Human Fc Capture (AHC) Biosensors	Sartorius	Cat# 18–5060
QuickChange XL II Kit	Agilent	Cat# 200521
TaqMan Copy Number Reference Assay, human, RNase P	Thermofisher	Cat# 4403326
Experimental models: cell lines
Raji	ATCC	CCL-86
293-6E cells	National Research Council, Canada	RRID:CVCL_HF20
293–2089	Delecluse et al., 1998	N/A
SVKCR2	Li et al., 1992b	RRID:CVCL_YD67
293T		RRID:CVCL_0063
AKATA-BX1-GFP	Molesworth et al., 2000)
Experimental models: Organisms/strains
NOD-scid Il2rgnull mice	The Jackson Laboratory	Stock No: 005557
Oligonucleotides
Forward primer specific for EBV BALF5 gene: CCCTGTTTATCCGATGGAATG	Kimura et al., 1999	N/A
Reverse primer specific for EBV BALF5 gene: CGGAAGCCCTCTGGACTTC	Kimura et al., 1999	N/A
FAM-labeled probe specific for EBV BALF5 gene: CGCATTTCTCGTGCGTGTACACC	Kimura et al., 1999	N/A
Recombinant DNA
BALF5 Target DNA for standard: ACCGAGACCCGGCAGGGGGTCCTGCGGTC GAAGGTGCTGGCCTTGAGGGCGCTGAGGA CTGCAAACTCCACGTCCAGACCCTGAGGC GCGCTGGCGTAGAAGTAGGCCTGCTGCCC AAACACGTTCACACACACGCTGGCCCCAT CGGCCTTGCGCCGGCCCAGTAGCTTGAT GACGATGCCACATGGCACCACATACCCCT GTTTATCCGATGGAATGACGGCGCATTTCT CGTGCGTGTACACCGTCTCGAGTATGTCG TAGACATGGAAGTCCAGAGGGCTTCCGTG GGTGTCTGCCTCCGGCCTTGCCGTGCCCT CTTGGGCACGCTGGCGCCACCACATGCCC TTTCCATCCTCGTCACCCCCCACCACCGTC AGGGAGTCTTGGTAGAAGCACAGGGGGGG CTGAGGCCCCCGCACATCCACCACCCCTGC GGCGCCTGGTGTCTGGAAACACTTGGGAAT GAGACGCAGGTACTCCTTGTCAGGCTTTTTC	Singh et al., 2020	Custom Synthesis Integrated DNA technologies
p2670	Delecluse et al., 1998	N/A
p509	Neuhierl et al., 2002	N/A
pTT3-gH-HIS-AVI	Snijder et al., 2018	N/A
pTT3-gL	Snijder et al., 2018	N/A
pTT3-gH	This Study	N/A
pTT3-gH-K73W-Y76A	This Study	N/A
pTT3-gH-IMX313	This Study	N/A
pTT3-gH-ferritin	This Study	N/A
pCVL-UCOE0.7-SFFV-muScn-IRES-GFP	Bandaranayake et al., 2011	N/A
pCVL-UCOE0.7-SFFV-gH-C153T-IMX313-IRES-GFP	This Study	N/A
pCVL-UCOE0.7-SFFV-gH-ferritin-IRES-GFP	This Study	N/A
pCVL-UCOE0.7-SFFV-gH--C153T-I3-IRES-GFP	This Study	N/A
pCVL-UCOE0.7-SFFV-gH-C153T-cTRP(6)ss-IRES-GFP	This Study	N/A
pCVL-UCOE0.7-SFFV-muScn-IRES-RFP	Bandaranayake et al., 2011	N/A
pCVL-UCOE0.7-SFFV-gL-IRES-RFP	This Study	N/A
psPAX2	Addgene	#12260
pMD2.G	Addgene	#12259
Software and algorithms
QuantaSoft™ Analysis Software	Bio-Rad	N/A
Prism 9.2.0 or later software package	Graph Pad Software	N/A
Leginon	Suloway et al., 2005	N/A
cryoSPARC	Punjani et al., 2017	N/A
CTFFIND	Rohou and Grigorieff, 2015	N/A
Appion	Lander et al., 2009	N/A
CTFFIND4	Mindell and Grigorieff, 2003	N/A
DoG picker	Voss et al., 2009	N/A
EMAN 1.9	Ludtke et al., 1999	N/A
RELION/2.1	Kimanius et al., 2016; Scheres, 2012a;	N/A
CryoSPARC	Punjani et al., 2017	N/A
CTFFIND4	Mindell and Grigorieff, 2003	N/A
ChimeraX	Pettersen et al., 2021	N/A
EPU	ThermoFisher	N/A
ASTRA	Wyatt Technologies	N/A
Other
QuantStudio 7 Flex Real-Time PCR System	Applied Biosystems	N/A
HiLoad 16/600 Superdex 200 pg	Millipore Sigma	Cat# GE28-9893-35
Superose 6 Increase 10/300 G	Millipore Sigma	Cat# GE29-0915-96
Hi-Trap Q HP	Millipore Sigma	Cat# GE29-0513-25
C1000 Touch Thermal Cycler	Bio-Rad	N/A
NGC FPLC	Bio-Rad	N/A
SpectraMax M2 plate reader	Molecular Devices	N/A
BD LSRII cytometer	BD Biosciences	N/A
Guava HT cytometer	Luminex	N/A
Octet Red 96E	Sartorius	N/A
1260 High-Performance Liquid Chromatography System	Agilent	N/A
Heleos multi-angle light scattering detector	Wyatt Technology	N/A
tREX refractive index detector	Wyatt Technology	N/A