Table 1.
Sequence comparison of the P. vivax proteins with their P. knowlesi orthologs
| P. vivax Protein | PlasmoDB code | # Hits P | Top Hit P | Synteny P | Similarity (%) P | Identity (%) P | Top Hit N | Identity (%) N |
|---|---|---|---|---|---|---|---|---|
| MSP8a | PVX_097625 | 1 | PKNH_1031500 | Y | 91.2 | 83.4 | AFL93300.1 | 84.52 |
| Pv-fam-aa | PVX_112670 | 24 | PKNH_1300800 | Y | 72.8 | 65.8 | OTN66803.1 | 83.11 |
| MSP1-19a | PVX_099980 | 1 | PKNH_0728900 | Y | 74.4 | 64.2 | AZL87433.1 | 81.31 |
| RAMA∗ | PVX_087885 | 0 | NA | NA | NA | NA | OTN68698.1 | 73.61 |
| RIPR | PVX_095055 | 1 | PKNH_0817000 | Y | 82.9 | 71.9 | XP_002258810.1 | 73.43 |
| Pv-fam-aa | PVX_096995 | 24 | PKNH_0200900 | Y | 50.9 | 41.1 | OTN66147.1 | 70.28 |
| PTEX150 | PVX_084720 | 1 | PKNH_0422900 | Y | 83.9 | 73.2 | OTN66840.1 | 72.02 |
| DBPII Sal 1 | PVX_110810 | 3 | PKNH_0623500 | Y | 64.7 | 52.1 | OTN68355.1 | 68.72 |
| DBPII AH | AAY34130.1b | 3 | PKNH_0623500 | Y | 64.7 | 51.9 | OTN68355.1 | 68.21 |
| Sexual-stage antigen s16 | PVX_000930 | 1 | PKNH_0304300 | Y | 78.6 | 67.1 | OTN68464.1 | 65.45 |
| TRAP | PVX_082735 | 1 | PKNH_1265400 | Y | 81.4 | 69.1 | AAG24613.1 | 64.23 |
| MSP3b | PVX_097680 | 15 | PKNH_1457400 | N | 42 | 28.6 | OTN63878.1 | 62.5 |
| MSP5 | PVX_003770 | 2 | PKNH_0414200 | Y | 65.4 | 49.9 | AAT77929.1 | 46.26 |
| Hypothetical | PVX_097715 | 0 | NA | NA | NA | NA | ANP24393.1 | 40.65 |
| EBP region IIa | KMZ83376.1b | 0 | NA | NA | NA | NA | QPL17772.1 | 35 |
| RBP2b1986–2653 | PVX_094255 | 2 | PKNH_0700200 | N | 52.2 | 29.1 | OTN67427.1 | 34.53 |
| MSP7F | PVX_082670 | 15 | PKNH_0726500 | N | 30.1 | 19 | AND94835.1 | 27.53 |
| RBP2b161–1454a | PVX_094255 | 2 | PKNH_0700200 | N | 52.2 | 29.1 | OTN67427.1 | 26.1 |
| RBP2a | PVX_121920 | 2 | PKNH_0700200 | N | 46.7 | 25.4 | OTN67427.1 | 25.77 |
| MSP7L | PVX_082700 | 0 | NA | NA | NA | NA | AND94835.1 | 24.29 |
| MSP3aa | PVX_097720 | 15 | PKNH_1457400 | N | 45.8 | 31.6 | NA | NA |
Full-length protein sequences were compared using the orthologs listed in PlasmoDB (H or A1H1 strain). The H strain and the A1H1 line gave identical results except for MSP7F; the H strain results are listed: A1H1 gave 11.5% identity and 18.8% similarity. Protein construct sequences (Table S1) were compared using NCBI BlastP (any strain) to identify the top hits. “P” denotes PlasmoDB pipeline, and “N” denotes NCBI pipeline. NA, no matches. Proteins are ordered by highest percent sequence identity using NCBI BlastP.
a
Top eight P. vivax protein serological-exposure marker.
b
GenBank IDs.