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. 2022 Jun 14;48(2):139. doi: 10.3892/or.2022.8350

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Detection of UBE2T-mediated ubiquitination of FANCI. (A) IP assay showed that UBE2T could bind to FANCI in A549 and H1299 cells. UBE2T exhibited higher (B) mRNA and (C) protein levels in NSCLC tumor tissues, compared with its levels in adjacent non-cancerous tissues. (D) Transfection efficiency of sh-UBE2T in NSCLC cells. (E) FANCI mRNA level did not change significantly upon UBE2T silencing. (F) Downregulation of UBE2T expression reduced FANCI levels. (G) The total FANCI protein from A549 and H1299 cells was obtained using the IP method. Compared with the ubiquitin content of the control group, the enrichment of ubiquitin increased after UBE2T downregulation. **P<0.01, ***P<0.001. UBE2T, ubiquitin-conjugating enzyme E2T; FANCI, Fanconi anemia complementation group I; IP, immunoprecipitation; sh, short hairpin; NS, not significant; NC, negative control.