Fig. 5.

End-guided meta-assembly accurately integrates single-cell data. A Performance of assemblers with input from increasing numbers of single mESC cells. Assemblies with a matching exon chain to a RefSeq transcript (left) or no match to a RefSeq transcript (right). B F-measure of assemblies, where recall is the proportion of all transcripts assembled by ≥1 strategy and precision is matches/total assemblies. C Comparison of Bookend meta-assembly to standard assembly and other meta-assemblers. Number of RefSeq-matching transcripts assembled (left) or the number of non-matches (right). D Precision/recall plot of the 12 assemblies from C; recall and precision calculated as in (B). E IGV browser image of the Commd8 gene. From top to bottom: RefSeq, Gencode, and Bookend mESC annotations, 5′ ends from mESC SLIC-CAGE, 3′ ends from mESC 3P-seq, Bookend-condensed partial assemblies from 96 single mESCs