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. 2022 Jun 16;13:927443. doi: 10.3389/fimmu.2022.927443

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Copyright © 2022 Sun, Zhang, Zhao, Yang, Shi, Xu, Liu, Guan, Shi and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of pPG-612-CK6-G/L.casei 393 and the protein expression. (A) Three-dimensional structures of recombinant CK6 and G proteins (B) Electrophoresis plots of amplification of different target genes, line 1: the G gene strip; line 3: the CK6 gene strip; line 5: the recombinant CK6-G strip; line 2, 4 and 6: blank control; (C) Double digestion electrophoresis plots of recombinant plasmids, 1: recombinant plasmid double digestion strip; M1: DNA marker DL5000, M2: DNA marker DL2000; (D) SDS-PAGE identification of protein expression of CK6-G gene, line 1: the pPG-612-CK6-G/L.casei 393 after induction; line 2: the uninduced pPG-612-CK6-G/L.casei 393; 3: blank control; M: 10-190 kDa protein marker (E) Indirect immunofluorescence map of the blank group. (F) Indirect immunofluorescence map of the control group. (G) Indirect immunofluorescence plot of the experimental group.