KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-mouse/human GATA3 | Cell Signaling Technology | Cat#5852S |
| Rabbit monoclonal anti-human PPM1D/WIP1 | Abcam | Cat#ab31270 |
| Rabbit monoclonal anti-human Phospho-p53 (Ser15) | Cell Signaling Technology | Cat#9284S |
| Rabbit monoclonal anti-human p53 | Cell Signaling Technology | Cat#9282S |
| Mouse monoclonal anti-human Phospho-ATM (Ser1981) | Cell Signaling Technology | Cat#4526S |
| Rabbit monoclonal anti-human/mouse ATM | Cell Signaling Technology | Cat#2873S |
| Mouse monoclonal anti-human c-Myc | Santa Cruz Biotechnology | Cat#SC-40 |
| Rabbit monoclonal anti-human/mouse CUL3 | Cell Signaling Technology | Cat#2759S |
| Rabbit monoclonal anti-human/mouse β-Actin | Cell Signaling Technology | Cat#4970S |
| Rabbit monoclonal anti-human IgG XP Isotype Control | Cell Signaling Technology | Cat#3900S |
| Mouse monoclonal anti DYKDDDDK Tag | Cell Signaling Technology | Cat#8146S |
| Rabbit monoclonal anti-HA-tag | Cell Signaling Technology | Cat#3724S |
| Goat anti-Mouse IgG Secondary Antibody, HRP | Thermo Fisher Scientific | Cat#31430 |
| Donkey anti-Rabbit IgG Secondary Antibody, HRP | Thermo Fisher Scientific | Cat#31458 |
| Rabbit monoclonal anti-human/mouse KIT | Cell Signaling Technology | Cat#3074 |
| Rabbit monoclonal anti-human/mouse CDH1 | Cell Signaling Technology | Cat#13116 |
| Rabbit monoclonal anti-human FOXA1 | Cell Signaling Technology | Cat#53528 |
| Mouse monoclonal anti-human KRT18 | Sigma Aldrich | Cat#WH0003875M1 |
| Rabbit monoclonal anti-human/mouse TP63 | Abcam | Cat#ab124762 |
| Rabbit monoclonal anti-human/mouse JAG1 | Cell Signaling Technology | Cat#70109 |
| Rabbit monoclonal anti-human FOXA1 | Cell Signaling Technology | Cat#53528 |
| Mouse monoclonal anti-human KRT14 | Santa Cruz | Cat#sc-53253 |
| Bacterial and Virus Strains | ||
| XL10-Gold Ultracompetent Cells | Agilent | Cat#200314 |
| Endura ElectroCompetent Cells | Lucigen | Cat#60242-2 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PBS | GIBCO | Cat#14190250 |
| DMEM, high glucose, pyruvate | GIBCO | Cat#11995065 |
| Lonza BioWhittaker L-Glutamine (200mM) | Lonza | Cat#BW17605E |
| Fetal bovine serum | VWR | Cat#9706 |
| Penicillin-Streptomycin | GIBCO | Cat#15140122 |
| PolyJet In Vitro DNA Transfection Reagent | SignaGen Laboratories | Cat#SL100688 |
| E-Gel Low Range Quantitative DNA Ladder | Invitrogen | Cat#NP0008 |
| E-Gel EX Agarose Gels, 2% | Life Technologies | Cat#G402002 |
| NuPAGE 3–8% Tris-Acetate Protein Gels, 1.5 mm, 10-well | Life Technologies | Cat#EA0378BOX |
| NuPAGE™ LDS Sample Buffer | Life Technologies | Cat#NP0008 |
| Pierce ECL Western Blotting Substrate | Thermo Fisher Scientific | Cat#32106 |
| Precision Plus Protein™ Dual Color Standards | Bio-Rad Laboratories | Cat#161-0394 |
| X-tremeGENE™ HP DNA Transfection Reagent | Sigma-Aldrich | Cat#6366236001 |
| Polybrene | Sigma-Aldrich | Cat#107689-10G |
| Puromycin dihydrochloride | Thermo Fisher Scientific | Cat#A1113803 |
| BamHI-HF | New England Biolabs | Cat#R3136S |
| EcoRI-HF | New England Biolabs | Cat#R3101S |
| FastDigest Esp3I | Thermo Fisher Scientific | Cat#FD0454 |
| Q5 DNA Polymerase | New England Biolabs | Cat#M0491L |
| Nuclease-Free Water | Ambion | Cat#AM9938 |
| Pierce™ Homobifunctional Cross Linkers | Life Technologies | Cat#20593 |
| 2-Mercaptoethanol | Sigma Aldrich | Cat#M6250-10ML |
| Dynabeads™ Protein A | Thermo Fisher Scientific | Cat#10004D |
| Dynabeads™ Protein G | Thermo Fisher Scientific | Cat#10002D |
| EDTA | Sigma Aldrich | Cat#E8008-100ML |
| Protease/Phosphatase Inhibitor Cocktail (100X) | Cell Signaling Technology | Cat#5872S |
| Quick-Load 1 kb Plus DNA Ladder | New England Biolabs | Cat#N0469S |
| LB Broth | Mp Biomedicals | Cat#244610 |
| L-Broth Agar Large Capsules | Mp Biomedicals | Cat#MP 113001236 |
| RIPA buffer | Invitrogen | Cat#R0278 |
| Pierce 16% Formaldehyde (w/v), Methanol-free | Life Technologies | Cat#28906 |
| Opti-MEM I Reduced Serum Medium, no phenol red | Thermo Fisher Scientific | Cat#11058021 |
| Cycloheximide powder | Cell Signaling Technology | Cat#2112 |
| Critical Commercial Assays | ||
| QIAprep Spin Miniprep Kit | QIAGEN | Cat#27106 |
| RNeasy Plus Mini Kit | QIAGEN | Cat#74134 |
| QIAquick PCR Purification Kit | QIAGEN | Cat#28104 |
| QIAquick gel extraction kit | QIAGEN | Cat#28704 |
| Gibson Assembly Master Mix | New England Biolabs | Cat#E2611L |
| iScript cDNA Synthesis Kit | Bio-Rad Laboratories | Cat#1708891 |
| SsoAdvanced Univ SYBR Grn Suprmx | Bio-Rad Laboratories | Cat#1725272 |
| Qubit dsDNA HS Assay Kit | Thermo Fisher Scientific | Cat#Q32854 |
| Qubit RNA HS Assay Kit | Thermo Fisher Scientific | Cat#Q32855 |
| GenElute™ HP Plasmid Maxiprep Kit | Sigma-Aldrich | Cat#NA0410-1KT |
| Ampure xp | Beckman Coulter | Cat#A63881 |
| BCA Assay Kit | Thermo Fisher Scientific | Cat#23225 |
| SMARTer® ThruPLEX® DNA-Seq Kit | Takara Bio | Cat#R400675 |
| Experimental Models: Cell Lines | ||
| Human: HEK293FT | Thermo Fisher Scientific | Cat#R70007 |
| Human: MDA-MB-231 | American Type Culture Collection | Cat#ATCC-HTB-26 |
| Human: CAL27 | Ravi Uppaluri lab | N/A |
| Human: CAL33 | Ravi Uppaluri lab | N/A |
| Deposited Data | ||
| GATA3 ChIP-Seq | This paper | GSE162003 |
| Original gel images | This paper | doi:10.17632/kgfzbpv2w4.1 |
| Oligonucleotides | ||
| Primers for PCR, see Table S5 | Invitrogen | N/A |
| Recombinant DNA | ||
| hWIP1-FLAG | Addgene | Addgene Plasmid #28105 |
| pHAGE-GATA3 | Addgene | Addgene Plasmid #116747 |
| lentiCRISPR v2 puro | Addgene | Addgene Plasmid #98290 |
| pMD2.G | Addgene | Addgene Plasmid #12259 |
| psPAX2 | Addgene | Addgene Plasmid #12260 |
| pHAGE-CMV-DsRed-IRES-GFP | Koren et al., 2018 | N/A |
| pHAGE-SFFV-GFP-IRES-DsRed | Timms et al., 2019 | N/A |
| pLenti-EF1a-PGK-Puro | Kai Wucherpfennig lab | N/A |
| pLenti-EF1a-GATA3-WT | This paper | N/A |
| pLenti-EF1a-GATA3-A442M | This paper | N/A |
| pLenti-EF1a-GATA3-G444E | This paper | N/A |
| pLenti-EF1a-GATA3-H400 | This paper | N/A |
| pLenti-EF1a-GATA3-WT-Fg | This paper | N/A |
| pLenti-EF1a-GATA3-A442M-Fg | This paper | N/A |
| pLenti-EF1a-GATA3-G444E-Fg | This paper | N/A |
| pLenti-EF1a-GATA3-H400-Fg | This paper | N/A |
| pLenti-EF1a-PPM1D-WT | This paper | N/A |
| pLenti-EF1a-PPM1D-V604Q | This paper | N/A |
| pLenti-EF1a-PPM1D-C605W | This paper | N/A |
| pLenti-EF1a-PPM1D-L450 | This paper | N/A |
| pLenti-EF1a-PPM1D-WT-HA | This paper | N/A |
| pLenti-EF1a-PPM1D-V604Q-HA | This paper | N/A |
| pLenti-EF1a-PPM1D-C605W-HA | This paper | N/A |
| pLenti-EF1a-PPM1D-L450-HA | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com |
| DeepDegron | This Paper | https://github.com/ctokheim/deepDegron |
| Transcription factor inference | This Paper | https://github.com/ctokheim/tf_association |
| Other | ||
| Corning Filter System (0.45um) | Corning Life Sciences | Cat#431096 |
| milliTUBE 1 ml AFA Fiber | Covaris Inc. | Cat#520130 |
| NITROCEL MEMB 0.45um | Bio-Rad Laboratories | Cat#1620115 |
| Multiplate™ 96-Well PCR Plates | Bio-Rad Laboratories | Cat#MLL9601 |
| QUBIT ASSAY TUBES SET | Life Technologies | Cat#Q32856 |
| Microseal ‘B’ Adhesive Seals | Bio-Rad Laboratories | Cat#MSB-1001 |