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. 2022 Jun 16;9:892763. doi: 10.3389/fmolb.2022.892763

FIGURE 5.

FIGURE 5

Comprehensive PTM map of COL1A1b of WT zebrafish heart ECM, presenting proline/lysine hydroxylation sites and lysine O-glycosylation sites. The representation of PTM sthe ites is similar to the COL1A1a PTM map as shown in top right corner. Peptides identified in proteomics analysis are shown in black color and unidentified peptides are shown in grey color. A total of 96.48% sequence coverage of COL1A1a is detected (considering the matured form of COL1A1b). The signal peptide is 22 amino acids (1–22) long. Sequence alignment matching and previous analysis by Gistelink et al. with human COL1A1 revealed the propeptide cleavage sites. Dark yellow arrows show N terminal (23–150) and C-Term (1,204–1,447) propeptide cleavage sites. Red bold “P” with a blue star represents 3-HyP and red “P” represents 4-HyP. Hydroxylysine is represented with bold “K” and yellow and blue circle represents the lysine O-glycosylation. A summary of these site-specific PTMs of COL1A1b is presented in Table 1, and all the PSMs for O-glycosylated lysine and 3-hydroxyproline sites are provided in Supplementary Figures. S2.22–S2.52.