TABLE 2.
Quantitative occupancy (%) of 3-hydroxyproline (3-HyP) sites identified in three different chains of collagen I (COL1A1a, COL1A1b, and COL1A2) from zebrafish cardiac ECM. Results are expressed as mean ± S.D. Significant statistical differences were estimated by ANOVA (*p < 0.05, nsp > 0.05).
| 3-HyP site | 3-Hydroxyproline average occupancy % | |||
|---|---|---|---|---|
| Sham average | 7 DPA Average | 14 DPA Average | 30 DPA Average | |
| COL1A1a P707 | 7.55 ± 4.3 | 31.49 ± 38.1 | 21.21 ± 7.8 | 20.50 ± 1.5 ns |
| COL1A1a P869 | 35.37 ± 6.9 | 22.82 ± 13.3 | 48.10 ± 63.8 | 20.12 ± 2.9 ns |
| COL1A1a P878 | 28.87 ± 7.2 | 17.57 ± 18.4 | 27.63 ± 35.8 | 12.76 ± 1.8 ns |
| COL1A1a P1148 | 48.73 ± 14.7 | 44.03 ± 17.0 | 68.96 ± 41.1 | 7.23 ± 4.6ns |
| COL5A2a P1195, 1201 | 1.77 ± 1.3 | 23.97 ± 4.5 | 55.24 ± 47.4 | 30.04 ± 41.1 ns |
| COL1A2 P718 | 0.19 ± 0.1 | 0.20 ± 0.3 | 0.10 ± 0.1 | 10.54 ± 7.4 ns |
| COL1A2 P925 | 5.14 ± 5.7 | 10.00 ± 13.2 | 0.53 ± 0.7 | 1.86 ± 1.8 ns |
| COL1A2 P1066 | 3.92 ± 3.4 | 3.87 ± 3.1 | 12.95 ± 15.7 | 6.91 ± 2.0 ns |
| COL1A2 P361 | 0.96 ± 0.3 | 1.52 ± 1.6 | 4.66 ± 4.5 | 1.18 ± 0.4 ns |
| COL1A1b P404 | 7.88 ± 1.9 | 18.28 ± 12.8 | 30.08 ± 6.7 | 11.95 ± 7.1 ns |
| COL1A1b P554 | 47.73 ± 4.0 | 46.52 ± 14.8 | 76.14 ± 29.7 | 42.91 ± 1.3 ns |
| COL1A1b P914 | 17.09 ± 1.4 | 16.74 ± 10.6 | 21.16 ± 14.0 | 47.35 ± 6.2 ns |
| COL1A1b P1031 | 12.44 ± 16.1 | 32.42 ± 34.0 | 18.31 ± 24.2 | 33.45 ± 3.6 ns |
| COL1A1b P1109 | 10.36 ± 7.6 | 3.93 ± 1.7 | 4.76 ± 6.0 | 32.64 ± 18.6 ns |