TABLE 3.
Quantitative micro-heterogenic occupancy (%) of O-glycosylated lysine sites identified three different chains of collagen I (COL1A1a, COL1A1b, and COL1A2) from zebrafish cardiac ECM. Results are expressed as mean ± SEM. Statistically significant differences were estimated by ANOVA (*p < 0.05, ns > 0.05). N/D, not detected; HyK, hydroxylysine; G-HyK, galactosyl-hydroxylysine; GG-HyK, glucosylgalactosylhydroxylysine.
| Microheterogeneity % | |||||
|---|---|---|---|---|---|
| Lysine sites | Modification | Sham | 7 DPA | 14 DPA | 30 DPA |
| COL1A1a K261 | K | 36.62 ± 9.7 | 43.75 ± 4.38 | 46.09 ± 4.89 | 32.30 ± 5.08 ns |
| HyK | 38.38 ± 10.38 | 34.81 ± 12.3 | 21.13 ± 2.85 | 45.72 ± 8.65 ns | |
| G-HyK | 16.71 ± 4.9 | 17.85 ± 12.0 | 31.26 ± 3.01 | 20.22 ± 3.01 ns | |
| GG-HyK | 8.28 ± 6.5 | 3.59 ± 4.3 | 1.32 ± 0.97 | 2.35 ± 0.57 ns | |
| COL1A1a K270 | K | 50.46 ± 5.3 | 32.92 ± 4.4 | 26.58 ± 1.7 | 46.48 ± 4.1 ns |
| HyK | 34.97 ± 0.7 | 37.13 ± 1.3 | 46.12 ± 1.1 | 40.69 ± 4.4 ns | |
| G-HyK | 12.26 ± 5.0 | 29.03 ± 4.4 | 24.56 ± 2.7 | 11.52 ± 0.4 ns | |
| GG-HyK | 2.32 ± 1.1 | 0.93 ± 1.3 | 2.74 ± 0.2 | 1.30 ± 0.7 ns | |
| COL1A1a K504 | K | 35.12 ± 0.2 | 6.54 ± 0.3 | 34.96 ± 46.9 | 51.61 ± 20.2 ns |
| HyK | 7.20 ± 4.4 | 3.49 ± 0.3 | 16.03 ± 22.4 | 16.85 ± 5.2 ns | |
| G-HyK | 57.68 ± 4.2 | 89.97 ± 0.0 | 49.01 ± 69.3 | 31.54 ± 15.0 ns | |
| GG-HyK | ND | ND | ND | ND | |
| COL1A1a K570 | K | 59.52 ± 12.5 | 24.79 ± 15.4 | 17.67 ± 1.6 | 42.84 ± 51.5 ns |
| HyK | 14.06 ± 4.6 | 6.83 ± 7.6 | 4.50 ± 0.1 | 0.21 ± 0.3 ns | |
| G-HyK | ND | ND | ND | ND | |
| GG-HyK | 26.42 ± 17.1 | 68.39 ± 7.8 | 77.83 ± 1.6 | 56.95 ± 51.8 ns | |
| COL1A1a K693 | K | 16.05 ± 1.8 | 11.35 ± 1.4 | 21.14 ± 15.4 | 17.46 ± 2.3 ns |
| HyK | 73.86 ± 2.2 | 83.40 ± 0.1 | 70.60 ± 23.8 | 74.25 ± 1.7 ns | |
| GK | 1.34 ± 0.3 | 0.25 ± 0.4 | 0.56 ± 0.8 | 1.47 ± 0.2 ns | |
| GGK | 8.75 ± 0.4 | 5.00 ± 1.0 | 7.69 ± 9.2 | 6.82 ± 3.8 ns | |
| COL1A1a K846 | K | ND | ND | ND | ND |
| HyK | ND | ND | ND | ND | |
| G-HyK | 48.69 ± 29.7 | 85.04 ± 19.4 | 51.02 ± 9.0 | 68.87 ± 5.5 ns | |
| GG-HyK | 51.31 ± 29.7 | 14.96 ± 19.4 | 48.98 ± 9.0 | 31.13 ± 5.5 ns | |
| COL1A1a K1017 | K | 62.44 ± 0.3 | 33.69 ± 22.8 | 52.55 ± 21.8 | 60.09 ± 0.5 ns |
| HyK | 34.82 ± 0.4 | 62.96 ± 20.9 | 36.95 ± 22.1 | 24.44 ± 0.2 ns | |
| G-HyK | ND | ND | ND | ND | |
| GG-HyK | 2.74 ± 0.7 | 3.35 ± 1.9 | 10.50 ± 0.3 | 15.47 ± 0.3 * | |
| COL1A1b K264 | K | 50.52 ± 15.5 | 40.63 ± 50.7 | 52.94 ± 15.8 | 20.39 ± 20.2 ns |
| HyK | 41.23 ± 8.2 | 53.16 ± 56.0 | 30.76 ± 34.6 | 46.65 ± 44.7 ns | |
| G-HyK | 5.19 ± 4.4 | 4.64 ± 3.6 | 9.75 ± 10.8 | 27.51 ± 20.7 ns | |
| GG-HyK | 3.05 ± 2.9 | 1.57 ± 1.6 | 6.54 ± 7.9 | 5.45 ± 3.7 ns | |
| COL1A1b K273 | K | ND | ND | ND | ND |
| HyK | 41.65 ± 47.5 | 50.93 ± 39.7 | 24.43 ± 25.6 | 33.90 ± 36.0 ns | |
| G-HyK | 5.58 ± 4.6 | 33.38 ± 20.8 | 27.55 ± 13.3 | 19.99 ± 10.5 ns | |
| GG-HyK | 52.77 ± 42.9 | 15.69 ± 19.0 | 48.02 ± 38.9 | 46.11 ± 25.5 ns | |
| COL1A1b K849 | K | 27.06 ± 1.1 | 10.35 ± 13.7 | 11.17 ± 2.2 | 22.95 ± 3.4 ns |
| HyK | 67.46 ± 1.1 | 68.53 ± 16.8 | 70.77 ± 9.7 | 62.89 ± 0.3 ns | |
| G-HyK | 3.35 ± 0.4 | 6.76 ± 9.4 | 12.97 ± 4.5 | 9.48 ± 1.5 ns | |
| GG-HyK | 2.13 ± 0.4 | 14.36 ± 12.4 | 5.09 ± 2.9 | 4.68 ± 2.2 ns | |
| COL1A2 K254 | K | 6.48 ± 3.2 | 20.75 ± 14.6 | 21.61 ± 14.3 | 55.69 ± 18.4 ns |
| HyK | 89.24 ± 1.6 | 78.24 ± 13.4 | 69.76 ± 2.2 | 43.90 ± 18.1 ns | |
| G-HyK | 0.11 ± 0.0 | 0.10 ± 0.1 | 0.22 ± 0.3 | 0.05 ± 0.0 ns | |
| GG-HyK | 4.17 ± 4.8 | 0.91 ± 1.1 | 8.40 ± 11.7 | 0.36 ± 0.3 ns | |
| COL1A2 K644 | K | ND | ND | ND | ND |
| HyK | 84.59 ± 1.8 | 61.15 ± 40.2 | 95.15 ± 6.5 | 90.77 ± 11.1 ns | |
| G-HyK | ND | ND | ND | ND | |
| GGK | 15.41 ± 1.8 | 38.85 ± 40.2 | 4.85 ± 6.5 | 9.23 ± 11.1 ns | |