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. Author manuscript; available in PMC: 2022 Jun 30.
Published in final edited form as: Methods Mol Biol. 2021;2275:315–327. doi: 10.1007/978-1-0716-1262-0_20

Figure 4. HPLC-MS/MS analysis of the media of neutrophil-like cells activated to produce hydrogen peroxide.

Figure 4.

To produce hydrogen peroxide, HL60 cells differentiated for 4 days with all-trans retinoic acid were stimulated with PMA (1 μM) in the presence and absence of catalase (1 kU/ml) and co-treated with o-MitoPhB(OH)2. Aliquots of media were collected after 30 min and processed and analyzed as described in the protocol. The intensities of o-MitoPhOH were multiplied by a factor of 5 and intensities of o-MitoPhNO2 were multiplied by 1000, to fit the same scale as of o-MitoPhB(OH)2.