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. 2022 Jun 27;5:602. doi: 10.1038/s42003-022-03536-0

Fig. 3. TAS-Seq detects more overall and highly variable genes than 10X v2 and Smart-seq2 in murine lungs with minimal technical variability.

Fig. 3

a Experimental scheme of TAS-Seq library generation from the lungs of adult C57BL/6 J mice. A lung single-cell suspension was obtained and processed using the BD Rhapsody workflow until exonuclease I treatment. Resulting beads were then split into three groups and TAS-Seq was performed individually. b and d Violin/box plot of the read number and detected gene number; scatter plot of the read number/detected gene number for each cell in TAS-Seq (deep-sequenced), 10X v2 (Tabula Muris), and Smart-seq2 datasets (b) or TAS-Seq (shallow-sequenced) and 10X v2 (GSM3926540) datasets (d). Box plot shows the mean of the read number with upper and lower quantiles, and the whisker shows ±1.5 × interquartile range. ****p = 0 (two-sided Wilcoxon rank-sum test). c and e The number of highly variable genes in TAS-Seq (deep-sequenced), 10X v2 (Tabula Muris), and Smart-seq2 datasets (c) or TAS-Seq (shallow-sequenced) and 10X v2 (GSM3926540) datasets (e). Exact p-values and statistics are shown in Supplementary Data 5.