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. 2022 Jun 28;17(1):2092699. doi: 10.1080/15592324.2022.2092699

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Interaction of SCaBP3 with AHA family members. (a). Evolutionary tree of the PM H⁺-ATPase family members of Arabidopsis thaliana by software MEGA. (b) Yeast two-hybrid assay of the interaction of PKS5 with AHA2C, as a positive control. (c) Yeast two-hybrid assay to analyze the interaction of SCaBP3 with AHA1C, AHA3C, AHA9C, AHA10C. (d) The negative control for the interaction of SCaBP3 with AHA1C, AHA3C, AHA9C, and AHA10C. For (b) and (c), yeast cells were observed and photographed after 3–5 days of growth on the corresponding medium.