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. 2022 Jun 28;17(1):2092699. doi: 10.1080/15592324.2022.2092699

Figure 2.

Figure 2.

The effect of SCaBP3 on PM H+-ATPase activity in RS-72 yeast. (a) 1645-SCaBP3 or 1645 empty vector was co-expressed with 1745-AHA2 full-length protein in yeast cells (RS-72). The samples were shaken at 28°C (220 rpm/min), and yeast growth (OD600) was measured every 30 min for 3 hours using a microplate reader. All data represent means ± standard deviations (SD) of at least five replicates.(b) 1645-SCaBP3 or 1645 empty vector was co-expressed with a truncated 1745-AHA2 protein lacking the C terminus (AHA2Δ92 and AHA2 Δ C) in yeast cells (RS-72). The samples were shaken at 28°C (220 rpm/min), and yeast growth (OD600) was measured every 30 min for 3 hours using a microplate reader. All data represent means ± SD of at least five replicates. (c) 1645-SCaBP3 or 1645 empty vector was co-expressed with a mutated 1745-AHA2 protein (AHA2P68S) in yeast cells (RS-72). The samples were shaken at 28°C (220 rpm/min), and yeast growth (OD600) was measured every 30 min for 3 hours using a microplate reader. All data represent means ± SD of at least five replicates. (d) Comparison of PM H+-ATPase-dependent growth of RS-72 yeast at 180 min. All data represent means values ± SD of at least five replicate experiments. A one-way ANOVA was used to determine statistical significance; significant differences (P ≤ .05) are indicated by different lowercase letters.