Table 2.

Secretomes from Ax4 grown in LB or VB were prepared in triplicate and subjected to mass spectrometry analysis.

	Putative Protein IDs	Unique peptides	Sequence coverage [%]	Fold change	Protein IDs
T6SS (TAX-1)	Colicin-E1–like effector	4	6.8	5.59	A0A0M7DM80
	VgrG1a	24	37	73.75	A0A0M7DL97
	Hcp1	13	75.4	6.92	A0A0D6HUB7;E3HG87
	VgrG1b + effector	4	2.8	12.04	E3HG94
	Effector domain	7	19.6	9.69	A0A0M7DKW2
	VgrG1c	32	47.6	50.74	A0A0M9I2Z4
	PAAR	3	8.8	5.31	A0A0M7DJN0
T3SS	CesT	4	29.6	8.53	A0A0M7IUG8
	SepL	11	35.2	11.15	A0A0M7KMF7
	EscI	6	42	2.85	A0A0M7KQZ8
Flagella	FliC	24	56.8	−3.00	A0A0M7L493
	FlgB	3	40.3	−2.83	A0A0M7M335;E3HSC4
	FlgC	9	89.9	−5.12	A0A0D6HEK3;E3HSC5
	FlgD	16	71.9	−3.95	A0A0M7KIR8;E3HSC6
	FlgE	14	45.5	−4.20	A0A0M7PS44;E3HSC7
	FlgF	12	64.8	−4.76	A0A0D6HCP9;E3HSC8
	FlgG	7	27.6	−5.31	A0A0M7M879;E3HSC9
	FlgK	18	37.5	−4.93	A0A0M7K095;E3HSD3
	FlgL	23	59.4	−4.67	A0A0D6HE69
	FliK	12	32.7	−5.23	A0A0M7F370;E3HSE7
	FliE	3	45.5	−4.65	A0A0D6HCW3;E3HSF3
	FliD	24	41	−2.87	A0A0M7D2V8
	FlaG	2	15.2	−6.12	A0A0M7NYY5

Protein included in TAX-1, T3SS, or flagella group are selected among significantly enriched in one growth condition.