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. 2022 Jun 23;119(26):e2121513119. doi: 10.1073/pnas.2121513119

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Copyright © 2022 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 5. — Interaction of Gnats with AChR. (A) Immunofluorescence confocal images showing the overlapping (yellow) expression of transducin (red) and AChR (green) in intact and Triton-treated ASMCs. (Scale bar, 10 μm.) (B–F) Coimmunoprecipitation of FLAG-MAChR M3 with HA-Gnat1, HA-Gnat2, HA-Gnat3, GFP-C-Gnat1 H5 domain and HA-Gnat1 DH5 domain in HEK293T cells. (G) Coimmunoprecipitation of endogenous MAChR M3 and Gnat1/Gnat2/Gnat3 in rat ASM tissues. (H–J) Calcium release in HEK293T cells transfected with the pCMV HA, HA-Gnat1, HA-Gnat1 H5 domain and HA-Gnat1 DH5 domain plasmids. Western blot and statistical analyses of transfected cells were performed. **P < 0.01, n > 7.