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TA 2D contour maps. (A–C) Original TA 2D spectra of Q48E, Q48A, and WT in H2O. (D–F) The corresponding TA 2D spectra of Q48E, Q48A, and WT after subtracting the contribution of FMN* from the original data in H2O. The transient species of FMN*, FMNH˙, 3FMN, and FMNred are denoted in the 2D contour maps.
