Fig. 3.

Expression of Minar2, localization of the protein in the cochlea and innervation of cochlear hair cells in Minar2 heterozygous mice, using the LacZ reporter gene component of the inserted cassette in the mutant allele, which expresses β-galactosidase (SI Appendix, Fig. S3). (A) RT-PCR of Minar2 expression in the cochlea at E18.5, P0, and P30. Also, expression in different mouse tissues at P30 are Hc, hippocampus; Cx, cortex; Li, liver; Sp, spleen; Ki, kidney; Lu, lung. Gapdh was used as a control. (B) Localization of Minar2 using the reporter gene LacZ of the mutant allele and in β-gal staining shown in blue. Note the localization of Minar2 at the SG, spiral ganglion; OC, organ of Corti. (Scale bar: 70 µm.) (C) Cross-section from 24-kHz region of P1 mutant inner ear was labeled with anti–β-gal (magenta) to detect Minar2 localization and Tuj1 (green) to label neurons. (Scale bar: 70 µm in C.) A zoom in is shown in D–F. (Scale bars: 10 µm.) IDC, interdental cells.