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. Author manuscript; available in PMC: 2022 Jun 30.
Published in final edited form as: Acta Biochim Pol. 2007 May 15;54(2):315–322.

Figure 1. Thiamine antagonists amprolium, oxythiamine and pyrithiamine affect viability of PC-12 cells in time- and dose-dependent manner.

Figure 1.

Viability of neuronally differentiated PC-12 cells was measured using WST-1 reagent and Multiscan RC (Thermolabsystems) upon treatment with 10, 100 or 1000 μM oxythiamine, pyrithiamine or amprolium in serum-reduced media for 48, 72 and 96 h. The viability rates represent the means ± S.E.M. for non-treated cells and cells treated with tested thiamine antagonists in which each value was determined from assays performed in quadruplicate. Further experimental details are given in Materials and Methods.