TABLE 1.
Summary of the genetic linkage map of P. ostreatus
| Marker type | Primera | Marker size (bp) or nameb (linkage group)c | Enzymed | Copy no.e | Chromosomef |
|---|---|---|---|---|---|
| RAPD | L1 | 1,075 (VI) | XhoI | Single | VI |
| 2,600 (X) | |||||
| RAPD | L2 | 1,850 (IV) | XhoI | Single | IV |
| 2,475 (XI) | |||||
| RAPD | L3 | 1,350 (IX) | PstI | Single | IX |
| RAPD | L4 | 2,400 (I) | |||
| RAPD | L5 | 875 (I) | Multiple | ||
| 1,475 (X) | XhoI | Single | X | ||
| 2,525 (IV) | |||||
| RAPD | L6 | 1,800 (IX) | PstI | Single | |
| RAPD | L7 | 825 (VII), 900 (I), 2,025 (I) | |||
| RAPD | L8 | 575 (IV) | Any oneg | Single | IV |
| RAPD | L10 | 2,175 (III), 2,925 (III) | |||
| RAPD | L11 | 1,325 (V), 1,450 (I) | |||
| RAPD | L13 | 2,575 (I) | |||
| RAPD | L14 | 1,525 (IX) | Multiple | ||
| 1,800 (II), 2,375 (III) | |||||
| RAPD | L15 | 625 (V) | XhoI | Single | V |
| 1,125 (I) | Multiple | ||||
| 1,275 (XI) | Multiple | ||||
| 1,525 (VI) | |||||
| RAPD | L16 | 875 (VII) | EcoRI | Single | VII |
| 1,100 (I) | PstI | Single | I | ||
| RAPD | L18 | 2,175 (I) | EcoRI | Single | I |
| 2,900 (II) | |||||
| RAPD | L19 | 1,750 (VII), 2,525 (IV) | |||
| RAPD | P1 | 750 (I), 1,600 (IX), 1,825 (VII) | |||
| RAPD | P2 | 725 (IX) | XhoI | Single | |
| 1,150 (IV), 2,100 (IX), 2,650 (IX) | |||||
| RAPD | P3 | 1,375 (IX), 1,550 (V) | |||
| RAPD | P4 | 600 (VI), 1,450 (III), 2,550 (VII) | |||
| RAPD | P5 | 1,225 (III) | |||
| RAPD | P6 | 950 (VI), 1,100 (X), 1,350 (N/A), 2,300 (XI) | |||
| RAPD | P7 | 1,275 (N/A), 1,525 (I) | |||
| RAPD | P8 | 1,875 (IV), 2,775 (X) | |||
| RAPD | P9 | 300 (N/A), 400 (VII), 875 (IV), 950 (II), 1,400 (VII), 1,500 (VII) | |||
| RAPD | P10 | 2,325 (VIII) | |||
| RAPD | P11 | 800 (V), 850 (III), 950 (IV) | |||
| RAPD | P12 | 950 (IX) | PstI | Single | IX |
| 1,350 (I) | |||||
| 1,525 (III) | Multiple | ||||
| 2,025 (II), 2,150 (VII), 3,525 (III) | |||||
| RAPD | P13 | 675 (IV), 1,250 (VI), 1,550 (V) | |||
| 1,625 (II) | |||||
| RAPD | P14 | 650 (VI) | |||
| RAPD | P15 | 950 (III), 1,050 (I), 1,225 (VIII) | |||
| RAPD | P16 | 400 (X), 1,525 (IX), 2,175 (II) | |||
| RAPD | P17 | 425 (II), 1,325 (VII), 1,425 (III) | |||
| RAPD | P19 | 525 (IX), 550 (III), 950 (II), 1,125 (II), 1,425 (VIII), 2,000 (VII), 2,100 (VI) | |||
| RAPD | R1 | 775 (I), 1,475 (VI) | |||
| RAPD | R2 | 1,600 (IX), 1,675 (V) | |||
| RAPD | R3 | 850 (III) | PstI | Single | |
| 925 (VI), 975 (III), 2,050 (VIII), 2,275 (IX), 2,475 (II), 3,625 (III) | |||||
| RAPD | R4 | 425 (VII), 450 (N/A), 925 (III), 1,175 (X), 2,425 (VIII), 3,150 (N/A) | |||
| RAPD | R6 | 400 (I), 950 (VI), 1,525 (II), 1,550 (II) | |||
| RAPD | R7 | 1,450 (X), 2,225 (IV), 2,400 (III), 2,700 (VII) | |||
| RAPD | R8 | 300 (VIII) | EcoRI | Single | VIII |
| 575 (III), 775 (VI), 1,475 (VI) | |||||
| RAPD | R9 | 1,300 (IV), 2,450 (X) | |||
| RAPD | R10 | 1,375 (V), 2,100 (VIII), 2,225 (VIII) | |||
| RAPD | R11 | 575 (VIII) | Multiple | ||
| 675 (III), 775 (I), 1,875 (VI) | |||||
| RAPD | R12 | 400 (I), 925 (VI), 1,475 (II), 1,500 (II) | |||
| RAPD | R13 | 525 (VII), 1,425 (IV), 2,175 (II) | |||
| RAPD | R14 | 700 (XI), 1,750 (VII) | |||
| RAPD | R15 | 575 (XI), 675 (IX), 1,025 (I), 1,200 (V), 1,750 (V), 3,100 (V) | |||
| RAPD | R16 | 775 (III), 975 (N/A), 1,025 (N/A), 1,200 (XI), 1,250 (II) | |||
| RAPD | R17 | 850 (VI), 1,500 (III) | |||
| RAPD | R19 | 1,300 (VII), 2,100 (III), 3,300 (XI) | |||
| RAPD | R20 | 650 (VIII) | EcoRI | Single | VIII |
| 1,400 (II), 1,500 (III), 1,950 (VIII), 2,700 (II) | |||||
| RAPD | S7 | 400 (VI) | |||
| 1,200 (V) | PstI | Single | V | ||
| 1,725 (II) | |||||
| RAPD | S11 | 975 (III) | PstI | Single | III |
| 2,325 (VIII) | |||||
| RAPD | S12 | 800 (XI), 1,550 (VIII), 2,075 (I), 2,425 (IV) | |||
| RAPD | S16 | 1,250 (X) | |||
| 1,275 (X) | Multiple | ||||
| RAPD | S17 | 775 (I), 825 (X), 2,375 (I) | |||
| RAPD | S18 | 1,175 (I) | |||
| 1,275 (III) | SmaI | Single | III | ||
| 1,925 (VII) | |||||
| RAPD | S19 | 1,250 (VII) | XhoI | Single | VII |
| 1,475 (VI), 2,600 (V) | |||||
| RFLP | O3 (X) | PstI | Single | X | |
| RFLP | O17 (II) | PstI | Single | ||
| RFLP | R3 (II) | PstI | Single | II | |
| RFLP | Hon2 (VI) | EcoRI | Single | ||
| RFLP | Rib (II) | XhoI | Single | II | |
| RFLP | fbh1 (XI) | EcoRI | Single | XI | |
| RFLP | vmh1 (I) | XhoI | Single | I | |
| RFLP | vmh2 (XI) | XhoI | Single | XI | |
| RFLP | vmh3 (X) | EcoRI | Single | X | |
| RFLP | pox1 (VI) | XhoI | Single | VI | |
| RFLP | poxC (VI) | XhoI | Single | VI | |
| RFLP | mnp1 (IV) | PstI | Single | IV | |
| RFLP | mnp3 (V) | HindIII | Single | V | |
| Mating | matA (III) | ||||
| Mating | matBα (IX) | ||||
| Mating | matBβ (IX) | ||||
| Isozyme | est1 (VIII) | ||||
| Repetitive DNA | rXhoI (II) | XhoI |
a
Operon oligonucleotide used as primer. RAPD markers are named after the primer oligonucleotide with the marker size as a subscript.
b
RAPD marker sizes are shown. RAPD markers transformed into RFLP markers are underlined. The names of RFLP, mating, isozyme, and repetitive DNA markers are those of the corresponding genes.
c
Indicates the linkage group where each marker maps. N/A, nonassigned markers.
d
Restriction enzyme used to reveal the RFLP markers used for linkage mapping.
e
Copy number of the corresponding RFLP marker. Only single-copy markers were used to construct the linkage map.
f
Physical mapping of the RFLP marker, confirmed by hybridization on PFGE, resolved the chromosomes of P. ostreatus N001.
g
Hemizygotic marker.