Skip to main content
. 2022 Feb 3;11(4):e024561. doi: 10.1161/JAHA.121.024561

Figure 5. cGAS/STING signaling activation is critical for the effects of microbial DNA.

Figure 5

A, The abundance of cGAS, phospho‐STING, and total STING in the adrenal glands of both normal chow diet (NCD) and 16 weeks high‐fat diet (16 weeks HFD) wild‐type (WT) mice. B, Effects of obese gut microbial DNA‐containing extracellular vesicles (mEVs) on the activation of cGAS/STING signaling in NCD CRIg−/− (complement receptor of the immunoglobulin superfamily) adrenal glands (B) or rat pheochromocytoma PC12 cells (C). The levels of adrenal inflammation (D), circulating catecholamine (E), and systolic blood pressure (SBP) (F) of 16 weeks HFD WT vs cGAS−/− mice. Effects of obese mEVs on the levels of SBP (G) of 16 weeks HFD cGAS−/− mice. H, The norepinephrine (NE) production of rat pheochromocytoma PC12 cells treated with obese mEVs and/or siRNA‐cGAS. Data are presented as mean±SEM. P values are determined by unpaired 2‐sided Student t test (A through F) or 1‐way ANOVA analysis (H). cGAS indicates Cyclic GMP–AMP synthase ; EPI, epinephrine; HSP90, heat shock protein 90; pSTING, phosphorylated STING; siRNA, small interfering RNA; and STING, the cyclic GMP–AMP receptor stimulator of interferon genes.