| Antibodies |
|
|
|
| anti-IE1, Mouse polyclonal |
Gift from Dr. Thomas Shenk |
N/A |
| Goat anti-Mouse Alexa Fluor 488 conjugate |
Invitrogen |
Cat# A-11001; RRID:AB_2534069 |
|
| Bacterial and virus strains |
|
|
|
| HCMV strain AD169 |
Gift from Dr. Thomas Shenk |
N/A |
| HCMV strain TB40/E |
Gift from Dr. Thomas Shenk |
N/A |
| HSV-1 strain 17+
|
Gift from Dr. Beate Sodeik |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Acyclovir |
Cayman Chemical |
Cat# 14160 |
| Cidofovir |
Cayman Chemical |
Cat# 13113 |
| CAY10602 |
Cayman Chemical |
Cat# 10009796 |
| DAPI |
ThermoFisher Scientific |
Cat# 62248 |
| HALT protease and phosphatase inhibitor |
ThermoFisher Scientific |
Cat# 78438 |
| TCEP |
ThermoFisher Scientific |
Cat# 77720 |
| Hygromycin |
ThermoFisher Scientific |
Cat# 10687010 |
| Sodium butyrate |
Sigma-Aldrich |
Cat# B5887 |
| Doxycycline |
Sigma-Aldrich |
Cat# D9891 |
| Polybrene |
Millipore Sigma |
Cat# TR-1003-G |
| SYBR green PCR master mix |
ThermoFisher Scientific |
Cat# 4368706 |
| Lipofectamine RNAiMAX |
ThermoFisher Scientific |
Cat# 13778150 |
|
| Critical commercial assays |
|
|
|
| BCA Assay |
ThermoFisher Scientific |
Cat# 23225 |
|
in situ Cell Death Detection Kit - Fluorescein |
Roche |
Cat# 1168479 |
|
in situ Cell Death Detection Kit - TMR red |
Roche |
Cat# 12156792910 |
| SuperScript IV First-Strand cDNA Synthesis Reaction Kit |
ThermoFisher Scientific |
Cat# 18091050 |
|
| Deposited data |
|
|
|
| PRM MS/MS data (Panorama) |
This study |
https://panoramaweb.org/HerpesvirusPRM.url
|
| PRM MS/MS data (RAW files) |
This study |
ProteomeXchange (PXD025879) |
|
| Experimental models: Cell lines |
|
|
|
| MRC5 |
ATCC |
Cat# CCL-171; RRID:CVCL_0440 |
| HEK293T |
ATCC |
Cat# CRL-3216; RRID:CVCL_0063 |
| U2OS |
ATCC |
Cat# HTB-96; RRID:CVCL_0042 |
| iSLK.219 |
Gift from Dr. Britt Glaunsinger |
N/A |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: BALB/cAnNTac |
Taconic Biosciences |
Cat# BALB-F |
|
| Oligonucleotides |
|
|
|
| CPS1 siRNA |
ThermoFisher Scientific |
Cat# EHU002511 |
| For qPCR primers, see Table S6
|
N/A |
N/A |
|
| Software and algorithms |
|
|
|
| Proteome Discoverer v2.4 |
ThermoFisher Scientific |
Cat# OPTON-31040 |
| Skyline |
(MacLean et al., 2010) |
https://skyline.ms/
|
| RawMeat |
Vast Scientific |
https://vastscientific.com/rawmeat
|
| Custom Python Code |
This study |
https://doi.org/10.5281/zenodo.6468269
|
