Fig. 1.

EGFP mRNA transfection in MH7A human fibroblasts. MH7A cells were transfected with in vitro-transcribed mRNA (IVT mRNA) and harvested at 24 h post transfection. a Scheme of the IVT mRNA design. b Western blotting of EGFP. c Intensity of fluorescence from MH7A cells in 96-well plates (n = 4, mean ± SEM). d Images of MH7A captured using a fluorescence microscope. Scale bar, 0.2 mm. e Cell viability was measured using the CellTiter-Blue Cell Viability Assay kit (n = 4, mean ± SEM). ARCA, anti-reverse cap analog; EGFP, enhanced green fluorescent protein; UTR, untranslated region