Fig. 3.

Multiplex assay of cytokines and chemokines in in vitro-transcribed mRNA (IVT mRNA)-transfected fibroblast-like synoviocytes (FLS) culture supernatant. The protein concentrations in the FLS culture supernatant were measured using a LEGENDplex Multi-Analyte Flow Assay kit. The samples contained humoral factors 24 h after transfection (n = 3). a IL-6 (pg/mL), b TNF-α (pg/mL), c CXCL10 (pg/mL), and d IL-8 (pg/mL) were detected. IL-1β, IFN-λ1, IL-12p70, IFN-α2, N-IFN-λ2/3, GM-CSF, IFN-β, IL-10, and IFN-γ were below detectable limits. Data are presented as mean ± SEM. t-test, *p < 0.05, **p < 0.01